Joyce lab

Our research program is focused on understanding how reciprocal communication between cancer cells and diverse immune and stromal cell types in the tumor microenvironment (TME) controls tumor initiation, progression, and metastasis, and modulates the response to therapeutic intervention. We employ a broad range of complementary experimental strategies to deeply interrogate the TME including the comprehensive analysis of patient samples (RNA-seq, proteomics, immune phenotyping, single cell analyses etc), mouse models of cancer, multi-modal in vivo imaging platforms, co-culture systems to mimic the TME, and diverse computational analyses. ...

Research projects

Tumor microenvironment

We currently have multiple projects focused on exploring the TME of primary and metastatic brain cancers, including analyses of diverse immune cell phenotypes, the blood and lymphatic vascular networks, and interrogation of the unique properties of the brain extracellular matrix. We are also fascinated by the dynamic evolution of the TME during cancer progression and metastasis in several tumor types, including breast, melanoma and lung cancer. Another major area of interest focuses on how different therapies alter the TME, and how those changes can consequently modulate therapeutic outcome. Our ultimate goal is to apply all of this knowledge to the clinic and develop targeted therapies that disrupt essential tumor-microenvironment interactions for the benefit of patients.

Selected Publications

Compensatory CSF2-driven macrophage activation promotes adaptive resistance to CSF1R inhibition in breast-to-brain metastasis.

Klemm F, Möckl A, Salamero-Boix A, (...), Plate KH, Joyce JA, Sevenich L

Nature cancer – 2021 Oct 18

An integrated pipeline for comprehensive analysis of immune cells in human brain tumor clinical samples.

Maas RR, Soukup K, Klemm F, (...), Daniel RT, Hegi ME, Joyce JA

Nature protocols – 2021 Aug 30

Therapeutic Targeting of the Tumor Microenvironment.

Bejarano L, Jordāo MJC, Joyce JA

Cancer discovery – 2021 Apr

Dynamic changes in glioma macrophage populations after radiotherapy reveal CSF-1R inhibition as a strategy to overcome resistance.

Akkari L, Bowman RL, Tessier J, (...), Westerga J, Watts C, Joyce JA

Science translational medicine – 2020 Jul 15

Interrogation of the Microenvironmental Landscape in Brain Tumors Reveals Disease-Specific Alterations of Immune Cells.

Klemm F, Maas RR, Bowman RL, (...), Daniel RT, Hegi ME, Joyce JA

Cell – 2020 May 28

Obesity alters the lung myeloid cell landscape to enhance breast cancer metastasis through IL5 and GM-CSF.

Quail DF, Olson OC, Bhardwaj P, (...), Holt PR, Dannenberg AJ, Joyce JA

Nature cell biology – 2017 Jul 24

Tumor-Associated Macrophages Suppress the Cytotoxic Activity of Antimitotic Agents.

Olson OC, Kim H, Quail DF, Foley EA, Joyce JA

Cell reports – 2017 Apr 4

The Microenvironmental Landscape of Brain Tumors.

Quail DF, Joyce JA

Cancer cell – 2017 Mar 13

Macrophage Ontogeny Underlies Differences in Tumor-Specific Education in Brain Malignancies.

Bowman RL, Klemm F, Akkari L, (...), Tabar V, Gutin PH, Joyce JA

Cell reports – 2016 Nov 10

STAT3 and STAT6 Signaling Pathways Synergize to Promote Cathepsin Secretion from Macrophages via IRE1α Activation.

Yan D, Wang HW, Bowman RL, Joyce JA

Cell reports – 2016 Sep 13

The tumor microenvironment underlies acquired resistance to CSF-1R inhibition in gliomas.

Quail DF, Bowman RL, Akkari L, (...), Holland EC, Sutton JC, Joyce JA

Science (New York, N.Y.) – 2016 May 20

Combined deletion of cathepsin protease family members reveals compensatory mechanisms in cancer.

Akkari L, Gocheva V, Quick ML, (...), Garfall AL, Bowman RL, Joyce JA

Genes & development – 2016 Jan 15

T cell exclusion, immune privilege, and the tumor microenvironment.

Joyce JA, Fearon DT

Science (New York, N.Y.) – 2015 Apr 3

Analysis of tumour- and stroma-supplied proteolytic networks reveals a brain-metastasis-promoting role for cathepsin S.

Sevenich L, Bowman RL, Mason SD, (...), Massagué J, Leslie CS, Joyce JA

Nature cell biology – 2014 Aug 3

CSF-1R inhibition alters macrophage polarization and blocks glioma progression.

Pyonteck SM, Akkari L, Schuhmacher AJ, (...), Holland EC, Daniel D, Joyce JA

Nature medicine – 2013 Sep 22